ANTI-FLAG® High Sensitivity, M2 coated 96-well plates
Кат. номер |
P2983-PH |
P2983-1EA |
P2983-100EA |
P2983-5EA |
The Anti-FLAG® HS, M2 Coated Plates are designed to ensure a higher binding capacity than other plates. This is due to the orientation of the Anti-FLAG® M2 mouse monoclonal IgG1 antibody on the plates. The antibody is covalently linked to the surface of a microtiter plate via the Fc portion of the antibody.
ANTI-FLAG® High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigmas GMP facility, ANTI-FLAG®P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.
Browse additional application references in our FLAG® Literature portal.
Once plates are opened, they should be stored with desiccant at 2- 8 °C and used within two weeks.
Components: The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
Coating:ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
Blocking: The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
Specificity:The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Sensitivity:Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capacity: Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
F-127 is a registered trademark of BASF SE
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Quality Level | 200 |
antibody product type | primary antibodies |
material | polystyrene |
clone | monoclonal |
shelf life | Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks. |
application(s) | ELISA: suitable |
isotype | IgG1 |
capacity | 100-300 ng/well |
storage temp. | 2-8°C |
Personal Protective Equipment | Eyeshields, Gloves |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |