N-Glycosidase F

Enzyme Commission (EC) Number:

3.5.1.52 ( BRENDA | IUBMB )

Кат. номер

11365193001

11365185001

N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.
Peptide-N-glycosidase F, Peptide-N4-(acetyl--glucosaminyl)-asparagine amidase
Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.
Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.
One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.
Clear, colorless solution after reconstitution
Storage conditions (working solution): 2 to 8 °C
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.
Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
assay	90% (SDS-PAGE)
form	lyophilized
specific activity	>25000 units/mg protein
packaging	pkg of 100 U (11365185001), pkg of 250 U (11365193001)
Торговая марка	Roche
optimum pH	7.0-8.0
storage temp.	2-8°C

RIDADR

NONH for all modes of transport