Anti-GAP43 Antibody, clone 1D6 , ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1220-4X25UL

ZRB1220-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 1D6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Growth-Associated Protein 43 (GAP43).
GST/His-tagged recombinant fragment corresponding to the first 126 amino acids from the N-terminal half of human Growth-Associated Protein 43 (GAP43).
Anti-GAP43, clone 1D6 ZooMAb, Cat. No. ZRB1220, is a highly specific rabbit recombinant monoclonal antibody that targets Growth-associated protein 43 (GAP43) and has been tested for use Immunocytochemistry, Immunohistochemistry (Paraffin). And Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representiatve lot detected GAP43 in human and mouse cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representiatve lot detected GAP43 in SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Target description
Neuromodulin (UniProt: P17677; also known as Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46) is encoded by the GAP43 gene (Gene ID: 2596) in human. GAP43 is a peripheral membrane protein that is associated with nerve growth. It serves as a common marker of differentiating neurons. It is a major component of the motile "growth cones" that form the tips of elongating axons and plays a role in axonal and dendritic filopodia induction. It is expressed at high levels in neuronal growth cones during development and axonal regeneration. However, it is not fond in dendritic cones. In adult brain, GAP43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. GAP43 contains an N-terminal lipid binding IQ domain (aa 31-60) that also binds calmodulin. GAP43 is reported to bind calmodulin at low calcium levels and then releases it when calcium levels increase indicating that calmodulin may serve as a negative regulator of GAP43 during periods of low neuronal activity. Protein kinase C-mediated phosphorylation serine 41 is reported to inhibit this interaction and stabilizes actin filaments and promotes growth cone extension. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Neve, RL et al., (1998). J. Neurosci. 18(19); 7757-7767).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1D6, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 24.8 kDa, observed mol wt ~47 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable